Portal InstitucionalUFPEL

01/04/2023 - 31/03/2027

Ciências da Saúde

The Kallikrein-Kinin System (KKS) participates in several regulatory functions in the body, highlighting inflammatory processes, blood clotting, and regulation of glucose homeostasis and blood pressure. This system is composed of two sets of substrates and enzymes, which generate the effector peptides called kinins. One of the sets is composed of circulating molecules such as the substrate kininogen, which is cleaved by kallikreins and other circulating enzymes, thus producing circulating kinins. The second set has similar factors produced in most tissues, such as tissue kininogen, tissue kalikreins, and other factors, which result in the production of kinins within tissues. The objective of this project is to verify the action of tissue kallikrein KLK1 on Mg ion homeostasis in the body. For this, a transgenic rat that expresses a greater amount of KLK1 will be compared with its controls, with regard to the serum, bone and urinary concentration of several important ions in the regulation of Mg, including, and in the expression of protein factors related to transport of these ions in the absorption in the intestine and in the secretion in the kidneys. This expression analysis will be done initially through qPCR to identify changes in mRNA expressions, and then by Western Blot, to verify changes in the effective amounts of these protein factors, as well as regulatory allosteric changes in these factors. As a result, we hope to identify new mechanisms of Mg regulation and its relationship with the action of KLK1 in renal tissues and in the intestine. This new knowledge may be important for the development of treatments for diseases related to imbalance in Mg metabolism in humans.